For those who exercise, thiamine may be a great additive to your supplement regiment, as it reduces ammonia and lactic acid build-up. This will help you squeeze out extra reps and recover faster during sets as well as to improve recovery post-workout. Optimal lactate and ammonia clearance will improve mental clarity (less brain fog), less inflammation, better sleep, better energy, etc… Start with 100mg and try working up to 1000mg thiamine HCL. Allithiamine would be the best, as it’s fat-soluble and stays much longer in your system (can last months) and the absorption is also much better, so smaller doses can be used. A good combination would be thiamine and sodium bicarbonate to decrease lactic acid and ammonia and increase pH.
Biosynthesis of steroid hormones requires a battery of oxidative enzymes located in both mitochondria and endoplasmic reticulum. The rate-limiting step in this process is the transport of free cholesterol from the cytoplasm into mitochondria. Within mitochondria, cholesterol is converted to pregnenolone by an enzyme in the inner membrane called CYP11A1. Pregnenolone itself is not a hormone, but is the immediate precursor for the synthesis of all of the steroid hormones. The following table delineates the enzymes required to synthesize the major classes of steroid hormones.
Current projects ongoing in Dr. Murray's laboratory include integrated approaches that assess the role of gap junctions and cell-to-cell communication in endocrine cell proliferation, migration, differentiation, and hormone production. Four different techniques (time-lapse video microscopy, immunocytochemistry, transmission electron microscopy, and western blot analysis) are being used to examine gap junction protein (connexin) transport, assembly, location and degradation. The effect of over expression and inhibition of gap junctions on adrenal cell function, are being studied with vectors containing cDNA antisense, dominant-negative constructs, siRNA approaches, mimetic peptides, and antibody directed against gap junction genes products. Together these studies are designed to elucidate the role connexin gap junction channels and cell-cell communication in regulating cell population response to physiological stimuli.