The steroids trenbolone acetate (TbA) and melengestrol acetate (MGA) are licensed as growth promoters for farm animals in several meat-exporting countries. Although many studies have explored their safety for both animals and consumers, little is known about their fate after excretion by the animal. Our study aimed to determine the residues and degradation of trenbolone and MGA in solid dung, liquid manure, and soil. In animal experiments lasting 8 weeks, cattle were treated with TbA and MGA. Solid dung and, in case of trenbolone, liquid manure were collected and spread on maize fields after and months of storage, respectively. Determination of the hormone residues in all samples included extraction, clean-up (solid-phase extraction), separation of metabolites and interfering substances by HPLC (RP-18), and quantification by sensitive enzyme immunoassay. Procedures were validated by mass spectrometry (MS) methods. During storage of liquid manure the level of trenbolone decreased from 1,700 to 1,100 pg/g (17alpha-isomer), corresponding to a half-life of 267 days. Before storage, the concentrations in the dung hill ranged from 5 to 75 ng/g TbOH and from to 8 ng/g MGA. After storage, levels up to 10 ng/g trenbolone, and 6 ng/g MGA were detected. In the soil samples trenbolone was traceable up to 8 weeks after fertilization, and MGA was detected even until the end of the cultivation period. The results show that these substances should be investigated further concerning their potential endocrine-disrupting activity in agricultural ecosystems.
Cellulose nitrate was once the standard film variety used by the motion picture industry — up until about the 1940s. That substance is unstable and highly flammable; cellulose nitrate fires are difficult to extinguish and produce dangerous gases, including corrosive nitrogen oxides and carbon monoxide. Many important films produced on cellulose nitrate have been forever lost due to degradative oxidation . More recent film stock employs cellulose acetate, the so-called "safety film." Unfortunately, even this improved film is subject to degradation, though it can be preserved for more than a century if stored under cold, dry conditions.
Sorry ladies, but Trenbolone is not recommended for you to use. Trenbolone is known for having virilization effects. It will lead to many things that happen with masculinization, including a deepening of the voice and hair growth. Hair growth can occur both on the body as well as on the face. Clitoral enlargement can also occur. It will also bind itself to progesterone receptors in the body, leading to the body increasing its prolactin levels. Lactation and breast growth can occur as a result at smaller amounts than the other side effects listed above. If you are serious about using Trenbolone and you want to reduce some of the side effects, then things like bromocriptine and Vitamin B6 can be used.